Review Paper Published in Annual Review of Analytical Chemistry
Researchers from the Center’s Soper group at the University of Kansas published a review paper in the June 2021 14th Volume of Annual Review of Analytical Chemistry. The paper is titled, “Analytical Technologies for Liquid Biopsy of Subcellular Materials.” Authors of the review are Camila D.M. Campos, Katie Childers, Sachindra S.T. Gamage, Harshani Wijerathne, Zheng Zhao, and Steven A. Soper.
Liquid biopsy markers, which can be secured from a simple blood draw or other biological samples, are used to manage a variety of diseases and even monitor for bacterial or viral infections. Although there are several different types of liquid biopsy markers, the subcellular ones, including cell-free DNA, microRNA, extracellular vesicles, and viral particles, are evolving in terms of their utility. A challenge with liquid biopsy markers is that they must be enriched from the biological sample prior to analysis because they are a vast minority in a mixed population, and potential interferences may be present in the sample matrix that can inhibit profiling the molecular cargo from the subcellular marker. In this article, we discuss existing and developing analytical enrichment platforms used to isolate subcellular liquid biopsy markers, and discuss their figures of merit such as recovery, throughput, and purity.