Past and Current Projects

**Table 1. New Collaborative Projects for Phase 3**
PI	New Phase 3 Collaborative Project Title	Affiliations
Patrick Fromal	Modular Mixed-Scale Systems for Detecting and Identifying Low Abundant Proteins from Clinical Samples	Shimadzu Corporation; Columbia, MD
Daniel Chiu	Extracellular RNA Carrier Subclasses in Processes Relevant to Substance use Disorders or HIV Infection	University of Washington, Seattle, WA
Stefanie Saupe	Patterning Ag/AgCl thin metal films on plastics for Resistive Pulse Sensing (RPS)	Natech Plastics Inc., Ronkonkoma, NY
Susan Lunte	COBRE: Molecular Analysis of Disease Pathways; National Institutes of Health	University of Kansas, Lawrence, KS
Mark Oldakowski	Integrated and Modular System for Sequencing DNA/RNA from Liquid Biopsy Samples	Bionano; San Diego, CA
Joanna Burdette	Dynamic Interactions of the Ovarian-Fallopian Axis in High Grade Serous Ovarian Cancer	University of Illinois, Chicago, IL
Vickie Wysocki	Native Mass Spectrometry Guided Structural Biology Center	Georgia Tech University, Atlanta, GA
Yen-Yu Ian Shih	SORDINO-fMRI for Mouse Brain Applications	UNC Chapel Hill, Chapel Hill, NC
Shilpa Buch	HIV Tat and Opiate-mediated aberrations in glial-neuronal crosstalk: Implications for the role of extracellular RNA in HAND	University of Nebraska Medical Center, Omaha, NE
Paul DeAngelis	Development of solid-state nanopore technology for improved glycosaminoglycan analytics	University of Oklahoma Health Sciences, Oklahoma City, OK

**Table 2. Continuing and New Additional Collaborative Projects**
PI	Continuing Collaborative Project Title	Affiliations
Prof. A. Godwin	Exosomes characterization in Ovarian Cancer	KUMC, Kansas City, KS
Dr. A. Baird	Integrated system for EVs analysis for Stroke diagnostic	SUNY Downstate Medical Center, New York City, NY
Mark Oldakowski	Commercialization of X-ToF sequencer for DNA	Bionano, Co, San Diego CA
Kambez Benam	Building Plastic MPS using Injection Molding	University of Pittsburgh, Pittsburg, PA
Jeanne Norton	Extrusion of COC 8007	Pittsburg State University, Pittsburg, KS
Kim Ritola	AAV status determination using RPS	UNC-Chapel Hill, NC
Kyoto Watanabe	Generating Plastic Chips for Electrophoresis	Shimadzu Corporation, Somerset, NJ
Jane McKeating	Surface Immobilization of XRN1 on Plastic Beads	University of Oxford, Oxford, UK
Jared Magoline	Patterning Nanostructures using Ion-Beam Free Methods	Teraprint, Chicago, IL
Gerson Aguirre	Plastic Nanofluidic chip for DNA stretching	Bionano, San Diego, CA
Dr. Taylor, Dr. Weir	Evs as a marker of bladder cancer	KUMC, Kansas City, KS
Dr. Navneet Dhillon	EV in neurological disorders	KUMC, Kansas City, KS
Dr. Shane Stecklein	EV miR sequencing for TNBC predicting treatment.	KUMC, Kansas City, KS
Dr. Frank Barone	Modelling stroke in animal models	SUNY Downstate, New York City, NY
Dr. Rolf Muller	Integrated system for selection/enumeration of VPs	BioFludica, San Diego, CA

**Table 3. Completed Past Collaborative Projects**
PI	Completed Past Collaborative Project Title	Affiliations
Dr. G. Gan	Isolation of CTCs from blood of head and neck squamous cell carcinoma (HNSCC) patients	KUMC, Kansas City, KS
Dr. Muller, Prof. Godwin, Prof. Soper, Dr. G. Gan	SARS CoV-2 Viral Particle Detection in patients hospitalized with COVID-19	Biofluidica, San Diego, CA
Dr. Belinda Sturm	Viral particles Isolation from Wastewaters	KU, Lawrence, KS
John Callahan	Sequencing bacterial pathogens at the point-of-use (POU)	Food and Drug Administration, Washington, DC
Daniel Chiu	Digital PCR Quantification of BCR-ABL for CML Diagnosis and Monitoring in a LMIC Setting	University of Washington, Seattle, WA
Heather Desaire	Development of a Rapid Glycosylation Profiling System using Mass Spectrometry Data	University of Kansas, Lawrence, KS
Subrajit Saha	Progenitor Cell Based Therapy to Mitigate Radiation Induced Gastrointestinal Syndrome	KU Medical Center, Kansas City, KS
Marc Porter	Field Deployable Platform for Prognostic Hepatic Cancer Screening in Low-Resource Settings	University of Utah, Salt Lake City, UT
Bruce Gale	High Throughput Fabrication of Nanopore Devices	University of Utah, Salt Lake City, UT
Sean Ford, Jeff Reifenberger, Mark Oldakowski	High Throughput Manufacturing of Saphyr Chip™	Bionano Genomics, San Diego, CA
Aaron Nagel	Searching for RNA Modifications	Digital NanoGenetics, Lawrence, KS
Keith August	Minimal Residual Disease (MRD) Monitoring using Peripheral Blood in Pediatric Patients with Acute Lymphoblastic Leukemia (ALL, T and B-type)	Children’s Mercy Hospital, Kansas City, MO
Dr. Victor Manneh	Modification of the surface of thermoplastics	Xen Biofluidx Inc., San Diego, CA
Lisa Podlaha-Murphy	Building highly durable mixed-scale electroformed molding tools	Clarkson University, Potsdam, NY
Alan Hamersley	High-scale production of nanometer structures using injection molding	Technicolor, Los Angeles, CA
Blake Peterson, Rich Givens	Photocleavable Linkers for Exosomes Release	University of Kansas, Lawrence, KS
Manas Gartia	Nanofluidic plasmonic sensors	Louisiana State University, Baton Rouge, LA
TJ Cradick	MPS for CRISPR Validation	CRISPR Technologies, Cambridge, MA
David Greenhalgh, Nam K	Mixed scale fluidic systems for the detection and characterization of the role and presence of bacteria during wound/burn healing	Shriner’s Hospital for Children, Sacramento, CA, UC Davis Pathology & Laboratory Med, Davis, CA
Taehyun Park, Kyungnam	PABP Thermofusion Bonding of High Tg Polymers	ULSAN University, Ulsan, South Korea
Anup Kasi	Circulating tumor cells as a potential biomarker in Colon and Appendiceal cancers with peritoneal metastasis	KU Cancer Center Kansas City, KS
Priyanka Sharma	Evaluation of longitudinal ctDNA to elucidate markers of response and acquired drug resistance to PI3K inhibitor therapy	KU Cancer Center, Kansas City, KS
Wen Jin Meng	Double-Punch Molding in Aluminum	Louisiana State University, Baton Rouge, LA
Tara Lin	Acute Myeloid Leukemia (AML) Circulating Leukemia Cells with Cancer Stem Cell Like Properties	KUMC and KU NCI Designated Cancer Center, Kansas City, KS
Matt Hupert	Integrated Systems for the Analysis of Circulating Multiple Myeloma Cells	BioFluidica, Chapel Hill, NC
George Martin, Terry Fetterhoff	Selection of CTC Orthogonal subpopulations	ROCHE, Pleasanton, CA
Lisa Carey	Enumeration of CTCs in metastatic breast cancer patients	University of North Carolina LCCC, Chapel Hill, NC
Young Whang	Enumeration of CTC in CRPC Patients	University of North Carolina LCCC, Chapel Hill, NC
Peter Voorhees	Analysis of CMMC for the Diagnosis of Multiple Myeloma	University of North Carolina School of Medicine, Chapel Hill, NC
Elizabeth Podlaha-Murphy	A New Platform for High Speed Sequencing of Single DNA Molecules	Northeastern University, Boston, MA
Tom Albert	Nanosensors for High Throughput Sequencing	ROCHE Diagnostics, Pleasanton, CA
JaeJong Lee	Molding/Demolding Technologies	Korea Institute of Machinery & Materials, Daejeon, South Korea
Seok-Yong Lee	Structure, Function, and Pharmacology of Neuronal Membrane Transport Proteins	Duke University, Durham, NC
Rolf Muller	Exosomes Isolation from Plasma of Breast Cancer Patients	Biofluidica, San Diego, CA
Rolf Muller	Isolation of cfDNA from Clinical Samples	Biofluidica, San Diego, CA
Prof. Paul Herring	Injection molding of thermoplastics	Pittsburg State University, Pittsburg, KS
Dr. Maria Bauer	Patterning Thin Film Conductors on Plastic Sheets	Stratec Consumables, Anif, Austria

**Table 1. New Service Projects for Phase 3**
PI	New Phase 3 Service Project Title	Affiliations/Funding
Paul DeAngelis	Development of solid-state nanopore technology for improved glycosaminoglycan analytics	University of Oklahoma Health Sciences, Oklahoma City, OK; National Institutes of Health; R01 GM134226
Dr. Neda Barjesteh, Dr. Stephane Shu Kin So	Isolation of cfDNA and EVs from animal samples. Zoetis	Corporate R&D funding; Kalamazoo, MI; www.zoetis.com
Alex Hyler	Microfluidic chips for the biophysical isolation of rare cells	CytoRecovery; Blacksburg, VA; Internal R&D funding
Jean-Luc Fraikin	Generating a production path for the Spectradyne consumable chip for RPS	Spectradyne, Signal Hill, CA; Corporate R&D funding
Anthony Atala, Sean Murphy	Human Organ Tissue Equivalent Models of Fibrosing Chemical Exposure to Bridge Preclinical and Clinical Translation of Medical Countermeasures	Wake Forest University School of Medicine, Winston-Salem, NC; Office of Assistant Secretary for Preparedness and Response/Biomedical Advanced Research and Development Authority (BARDA)
James Keck	Generation of Fusion Proteins For DNA Repair	University of Wisconsin, Madison; 2RM1GM130450
Greg Cooksey	Plastic-based microfluidic chips for the analysis of single cells using flow cytometry	National Institute of Standards and Technology; Gaithersburg, MD; NIST R&D Funding
David Issadore	High Throughput Digital Droplet Assays for Ultrasensitive Multimodal (DNA, RNA, and Protein) Diagnostic	University of Pennsylvania; R33CA287135
Susanta Hui	Myeloid cell-selective, oligonucleotide-based STAT3 inhibition combined with total marrow and lymphoid irradiation for immunotherapy of acute myeloid leukemia	City of Hope, Duarte, CA; 5R01CA284593-02
Greg Studdard	New chip for the sorting of EVs in a label-free fashion	BioFluidica, Durham, NC; Corporate R&D funding

**Table 2. Continuing and New Additional Service Projects**
PI	Continuing Service Project Title	Affiliations
Prof. Joanna Burdette	Selection of ovarian cancer associated EVs	University of Illinois, Chicago, Il
Prof. Martin	Fabrication of Microfluidic Molds	Univ. of St. Louis, St. Louis, MO
Prof. John McDevitt	Development of plastic chips for oral cancer diagnostics	Oraliva New York, NY
Prof. Aaron Timperman	Fabrication and molding of structures for An Integrated Microfluidic System for Combining Top-Down and Bottom-Up Proteomics	University of Illinois, Urbana-Champaign, IL
Prof. Jason Springer	Development of a chip-based analytical platform to enable point-of-care monitoring of peripheral rituximab levels	Vanderbilt University Medical Center, Nashville, TN
Prof. Vivien Coulson-Thomas	Age-related changes in HA in the context of Meibomian gland dysfunction	College of Optometry, University of Houston, Houston, TX
Prof. Francesca E. Duncan	Analysis of follicular fluid HA size distribution to probe the role of matrix degradation on oocyte expansion in reproductive aging in humans	Feinberg School of Medicine, Northwestern University, Boston, MA
Prof. Kelley, S. O.	Tumor Cell Isolation	Cellular Analytics, Toronto, Canada
Dr. Julie Hakim	Nanopore analysis of hyaluronan size distribution	Baylor College of Medicine, Waco, TX
Dr. Aguirre Gerson	Building Plastic Devices for DNA Stretching	BionanoGenomics, San Diego, CA
Try Shoop	Generating new plastics for high process yield rate of nanofluidic devices	Polyplastics USA, Inc., Farmington Hills MI
Dave Alberty	Processing large sample volumes for isolation of EVs	InnovaPrep, Drexel, MO
Prof. Kambez Benam	Building plastic MPS using injection molding	University of Colorado, Boulder, CO
Dr. Stavros Garantziotis	Nanopore analysis of HA size distribution	National Institute of Environmental health Sciences, Durham, NC

**Table 3. Completed Past Service Projects**
PI	Completed Past Service Project Title	Affiliations
Prof. Pulliam, Lynn	Brain-derived exosomes as blood markers of HIV cognitive impairment	Northern California Institute Research & Education, San Francisco, CA
Dr. Kasi, Anup MD	Circulating Tumor Cells for integrating PARP inhibitors in DNA repair-deficient Pancreatic Cancers	KU Medical Center, Kansas City, KS
Dr. Yipeng Wang	CTC isolation in met PDAC patients enrolled in NIRA-PANC clinical trial	KU Medical Center, Kansas City, KS
Dr. Hyler, Alex	Microfluidic chips for the biophysical isolation of rare cells	CytoRecovery (Blacksburg, VA)
Prof. Dominique de Kleijn, Dr. Egbert Smit	Evaluation of suitability of affinity chip for PERSUASIVE's EV analytical and clinical diagnostic workflow	Persuasive Dx, UMC Utrecht, The Netherlands, Europe
Prof. Polineni, Deepika MD	Nasal Lavage Exosome Analysis Specifies Therapeutic Targets for Precision Treatment in Cystic Fibrosis	KU Medical Center, Kansas City, KS
Dr. Morris, Jill K.	Exosomes characterization in Alzheimer's	KU Medical Center, Kansas City, KS
Prof. Sharma, Priyanka	Evaluation of longitudinal ctDNA to elucidate markers of response and acquired drug resistance	KU Medical Center, Kansas City, KS
Dr. Funk, Ryan	Development of chip-based assay for the quantitation of molecular markers of MTX response in autoimmune arthritis	KU Medical Center, Kansas City, KS
Dr. Ford, Shawn		Bionano, Co. San Diego, CA
Prof. Heungjoo Shin	Development of a Multiplex Biosensor Array Chip Passed on Stacked Triple Carbon Nano-electrode Sets for the Early Diagnosis of Myocardial Infarction and Glucose Monitoring	UNIST (Ulsan National Institute of Science & Technology)
Prof. Hatim Husain, Rolf Muller	CTC in Lung Cancer	Biofluidica, Inc
Prof. Ganguly, Sid	Isolation of CPC from Blood of Patients Diagnosed with Plasma Cell Disorders	KU Medical Center, Kansas City, KS
Prof. Armistead, Paul	Isolation of AML CLC	University of North Carolina, Chapel Hill, NC
Prof. Bae-Jump, Victoria	Isolation of CTC in Epithelial Ovarian Cancer	University of North Carolina, Chapel Hill, NC
Prof. Jarrod A. Marto	Fabrication of plastic chips via injection molding	Dana Farber Cancer Institute, MA
Dr. Teddy de Groot	Fabrication of metal mold	LevitasBio, CA
Dr. Vivien Coulson Thomas	Nanopore analysis of hyaluronan size distribution	University of Houston
Dr. Francesca Duncan	Nanopore analysis of hyaluronan size distribution	Northwestern University
Dr. Steven P. Jones	Nanopore analysis of hyaluronan size distribution	University of Louisville
Dr. Larry Sherman	Nanopore analysis of hyaluronan size distribution	Oregon Health & Science University
Dr. Oliver Porquie	Nanopore analysis of hyaluronan size distribution	Harvard Medical School
Dr. Rebecca Wells	Nanopore analysis of hyaluronan size distribution	University of Pennsylvania
Dr. Michele T. Pritchard	Nanopore analysis of hyaluronan size distribution	University of Kansas Medical Center
Dr. Sabina Hrabetova	Nanopore analysis of hyaluronan size distribution	SUNY Downstate Health Sciences University
Dr. Jennifer Curtis	Nanopore analysis of hyaluronan size distribution	Georgia Tech
Dr. Arthur Beaudet	Fetal Cell Capture from Blood of Pregnant Women	Luna Genetics, TX
Prof. Keith August	Circulating Leukemic Cells Detection in B-Cell ALL	Children’s Mercy Hospital, Kansas City, MO
Dr. Don Owens	Nanopore analysis of hyaluronan size distribution	TFF Pharmaceuticals, Foxboro, MA
Greg Gan	Isolation of CTCs from blood of head and neck squamous cell carcinoma (HNSCC) patients	KU Medical Center, Kansas City, KS
Prof. Ashleigh Theberge	Injection Molding of Microstructures	University of Washington, Seattle, WA
Prof. Jen Jen Ye	CTC in PDAC	University of North Carolina, Chapel Hill, NC
Prof. Charles Perou	CTC in Breast Cancer	University of North Carolina, Chapel Hill, NC
Matt Milowski	CTC in CRPC	University of North Carolina, Chapel Hill, NC
Prof. Victoria Bae-Jump	Circulating Tumor Cells in Endometrial Cancer Patients	University of North Carolina, Chapel Hill, NC
Prof. Vinod Dasa, Jeffery Hobden	Microfluidics for the Analysis of Bacterial Pathogens	LSU HSC, New Orleans, LA
Prof. Deok-ho Kim	Development of 3D Cell Culture Surfaces	University of Washington, Seattle, WA
Prof. In-Hyouk Song	Development of Device for Bacterial Cells Isolation	Texas State University, San Marcos, TX
Prof. Dorel Moldovan, Dimitris Nikitopoulos, MD	Simulations of Transport in Nano-confined Environments	LSU, Baton Rouge, LA
Prof. Kidong Park	Developing roll-to-roll process for cell culture substrate	LSU, Baton Rouge, LA
Dr. Margaret Gulley	Cell-free DNA in blood from cancer patients	University of North Carolina, Chapel Hill, NC
Dr. Gerard J. Oakley III	Phase 1b/2 Study of Olaratumab plus Doxorubicin in Soft Tissue Sarcoma (STS) Using CTCs as a Companion Biomarker	Eli Lilly and Company, Indianapolis, IN
Prof. Susan Lunte	Microfluidic Methods for Measurement of Mitochondrial Biomarkers of Alzheimer’s disease	University of Kansas, Lawrence, KS
Prof. Linheng Li	Hematopoietic Stem Cell Isolation	Stowers Institute for Medical Research, Kansas City, MO