Two members of the CBM2 team participated in the
March 17-21 PITTCON 2019 in Philadelphia, PA.
Charuni Amarasekara, a graduate student in the Soper
research group, presented her poster “Electrokinetic Separation of Single
Stranded Oligonucleotides in Thermoplastic Nanochannels” on Tuesday, March 19,
2019 in the Capillary Electrophoresis poster session. Authors on the poster
were Charuni Anuradha Amarasekara, Steven Allan Soper and Kumuditha M Weerakoon-Ratnayake.
Prof. Steven A. Soper presented “Nanosensors for
Single-Molecule Sequencing” on Wednesday, March 20, 2019. The abstract to Dr.
Soper’s talk is:
We are generating a single-molecule DNA sequencing platform
that can acquire sequencing information with high accuracy. The technology
employs arrays of nanosensors that read the identity of individual
mononucleotides from their characteristic electrophoretic mobility and current
transient amplitudes through a 2-dimensional (2D) nanochannel (~20 nm in width
and depth; <10 µm in length) fabricated in a thermoplastic via
nano-imprinting (NIL). The mononucleotides are generated from an intact DNA
fragment using a highly processive exonuclease, which is covalently anchored to
a plastic solid support contained within a bioreactor that sequentially feeds
mononucleotides into the 2D nanochannel. We will discuss the operation of this
nanosensor in this presentation as well as its production.